C02 - Electrical and mechanical stimulation of hyaline cartilage: Characterisation of biological response and stimulation parameters

Summary of the work programme

Within this project, the influence of electrical and mechanical stimulation on chondrogenic differentiation of human cartilage cells and mesenchymal stem cells will be analysed in vitro. For this purpose, we will use a specific stimulation chamber for experimental analyses of single and combined stimulation. Further, we will establish a multiphysics simulation model which should comprise the stimulation parameters and their influence on cells resulting in an enhanced understanding of the stimulation process. In the first funding period, the main goal of C02 is to establish and achieve optimised 3D cultivation of human chondrocytes and mesenchymal stem cells on novel collagen- and hydrogel-based biomaterials (scaffolds) by means of combined electrical and mechanical stimulation to derive effective therapeutic approaches.

Cooperation projects

A02 / A03 / A05 / B01 / B02 / B03 / C01 / C03 / C04

Publications

2019

Cachão, João Henrique; Soares Dos Santos, Marco P.; Bernardo, Rodrigo; Ramos, António; Bader, Rainer; Ferreira, Jorge A. F. et al. (2019): Altering the Course of Technologies to Monitor Loosening States of Endoprosthetic Implants. In: Sensors 20 (1). DOI: 10.3390/s20010104.

Farooqi, Abdul Razzaq; Bader, Rainer; van Rienen, Ursula (2019): Numerical Study on Electromechanics in Cartilage Tissue with Respect to Its Electrical Properties. In: Tissue engineering. Part B, Reviews 25 (2), S. 152–166. DOI: 10.1089/ten.teb.2018.0214.

Farooqi, Abdul Razzaq; Zimmermann, Julius; Bader, Rainer; van Rienen, Ursula (2019): Numerical Simulation of Electroactive Hydrogels for Cartilage-Tissue Engineering. In: Materials 12 (18). DOI: 10.3390/ma12182913.

Krüger, Simone; Achilles, Sophie; Zimmermann, Julius; Tischer, Thomas; Bader, Rainer; Jonitz‑Heincke, Anika (2019): Re-Differentiation Capacity of Human Chondrocytes in Vitro Following Electrical Stimulation with Capacitively Coupled Fields. In: Journal of clinical medicine 8 (11). DOI: 10.3390/jcm8111771.

Raben, Hendrikje; Kämmerer, Peer W.; Bader, Rainer; van Rienen, Ursula (2019): Establishment of a Numerical Model to Design an Electro-Stimulating System for a Porcine Mandibular Critical Size Defect. In: Applied Sciences 9 (10), S. 2160. DOI: 10.3390/app9102160.

Rohde, Marco; Ziebart, Josefin; Kirschstein, Timo; Sellmann, Tina; Porath, Katrin; Kühl, Friederike; Delenda, Bachir; Bahls, Christian; van Rienen, Ursula; Bader, Rainer; Köhling, Rüdiger (2019): Human Osteoblast Migration in DC Electrical Fields Depends on Store Operated Ca2+-Release and Is Correlated to Upregulation of Stretch-Activated TRPM7 Channels. In: Frontiers in bioengineering and biotechnology 7, S. 422. DOI: 10.3389/fbioe.2019.00422.


2018

Hiemer, Bettina; Krogull, Martin; Bender, Thomas; Ziebart, Josefin; Krüger, Simone; Bader, Rainer; Jonitz‑Heincke, Anika (2018): Effect of electric stimulation on human chondrocytes and mesenchymal stem cells under normoxia and hypoxia. In: Molecular Medicine Reports 18 (2), S. 2133–2141. DOI: 10.3892/mmr.2018.9174.

Kluess, Daniel; Lange, Hans-E.; Heyer, Horst; Sander, Manuela; Mittelmeier, Wolfram; Bader, Rainer (2018): Supplementary finite element analysis in experimental testing of total hip stems. In: Materials Testing 60 (5), S. 489–494. DOI: 10.3139/120.111182.

Ziebart, Josefin; Fan, Siyuan; Schulze, Christian; Kämmerer, Peer W.; Bader, Rainer; Jonitz‑Heincke, Anika (2018): Effects of interfacial micromotions on vitality and differentiation of human osteoblasts. In: Bone & Joint Research 7 (2), S. 187–195. DOI: 10.1302/2046-3758.72.bjr-2017-0228.r1.

Conference papers

2020

Plocksties, Franz; Niemann, Christoph; Bader, Rainer; Möws, Fabian; Seitz, Hermann; Kämmerer, Peer W. et al. (2020): Requirements and Design of an Implant for Electrical Stimulation for Bone Regeneration in Animals. IEEE Conference on Life Sciences and Technologies (LifeTech). Kyoto, Japan, March 2020.
 

2019

Krüger, Simone; Achilles, Sophie; Jonitz‑Heincke, Anika; Bader, Rainer (2019): In vitro Charakterisierung humaner Chondrozyten unter Stimulation mit elektrischen Wechselfeldern verschiedener Frequenzen. Deutscher Kongress für Orthopädie und Unfallchirurgie. Berlin, Germany, October 2019.

Sahm, Franziska; Grote, Vivica Freein; Detsch, Rainer; Krelel, Thomas; Boccaccini, Aldo R.; Bader, Rainer; Jonitz‑Heincke, Anika (2019): Influence of low-frequency alternating electrical fields on the bone remodeling capacity of human osteoblast. European Orthopaedic Research Society. Maastricht, Netherlands, October 2019.

Weizel, Alina; Distler, Thomas; Detsch, Rainer; Boccaccini, Aldo R.; Seitz, Hermann (2019): Relaxation Behaviour of Ionically and Enzymatically Microbial Transglutaminase (mTG)-Crosslinked Alginate-di-aldehyde and Gelatin (ADA-GEL) Hydrogels for Cartilage Regeneration. 4th Euro Intelligent Materials 2019. Kiel, Germany, June 2019.

Weizel, Alina; Zimmermann, Julius; Riess, Alexander; Krüger, Simone; Bader, Rainer; van Rienen, Ursula; Seitz, Hermann (2019): Numerical simulation of the electric field distribution in an electrical stimulation device for scaffolds settled with cartilaginous cells. 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). Berlin, Germany, July 2019. doi:10.1109/EMBC.2019.8857760


2018

Möws, Fabian; Ziebart, Josefin; Gabler, Carolin; Krüger, Simone; Wolter, Daniel; Ellenrieder, Martin; Bader, Rainer (2018): Etablierung eines Tiermodells zur Untersuchung der Osseointegration elektrostimulierender Implantate. VIII. Münchener Symposium für Experimentelle Orthopädie, Unfallchirurgie und Muskuloskelettale Forschung. Munich, Germany, March 2018.

Talks

2017

Bader, Rainer (2017): Numerische Simulation in der Biomaterialforschung. University Medical Center. Rostock, Germany, November 2017.

Posters

2019

Bansod, Yogesh Deepak; Kebbach, Märuan; Bader, Rainer; van Rienen, Ursula (2019): A Piezoelectric Strain-Adaptive Bone Remodeling Simulation. 41st International Engineering in Medicine and Biology Conference. Berlin, Germany.

Farooqi, Abdul Razzaq; Zimmermann, Julius; Bader, Rainer; van Rienen, Ursula (2019): Numerical Simulation of Electro-Active Hydrogels to Be Used for Cartilage Tissue Engineering. 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). Berlin, Germany.

Weizel, Alina; Distler, Thomas; Polley, Christian; Detsch, Rainer; Boccaccini, Aldo R. (2019): Mechanical and rheological characterization of hydrogels and hydrogel precursors for cartilage tissue engineering and biofabrication. Additive Manufacturing Meets Medicine + 3D Week. Lübeck, Germany.

Weizel, Alina; Distler, Thomas; Polley, Christian; Detsch, Rainer; Boccaccini, Aldo R.; Seitz, Hermann (2019): Relaxation Behavior of Ionically and Enzymatically Microbial Transglutaminase (mTG)-Crosslinked Alginate-di-aldehyde and Gelatin (ADA-GEL) Hydrogels for Cartilage Regeneration. 4th Euro Intelligent Materials. Kiel, Germany.

Patents

2019

Bader, Rainer; Bender, Thomas; Jonitz‑Heincke, Anika (2019): Implantatanordnung zur elektrischen Stimulation von Knorpelgewebe. Angemeldet durch Universität Rostock am 23.05.2018. Veröffentlichungsnr: DE 10 2018 112 297 A1.

Bader, Rainer; Bender, Thomas; Jonitz‑Heincke, Anika; Seitz, Hermann; Lieberwirth, Clemens; Riess, Alexander (2019): Vorrichtung zur elektrischen und mechanischen Stimulation von Gewebeproben. Angemeldet durch Universität Rostock. Veröffentlichungsnr: DE 10 2018 114 019 A1.

Bader, Rainer; Bender, Thomas; Ziebart, Josefin; Vogel, Danny (2019): Vorrichtung zur Behandlung und/oder Vermeidung von Biofilmen und/oder Infektionen an permanent bestehenden oder temporär bestehenden Hautdurchtritten. Angemeldet durch Universität Rostock am 23.05.2018. Veröffentlichungsnr: DE 10 2018 112 298 A1.

Project leader

Prof. Dr. med. habil. Dipl.-Ing. Rainer Bader
E-Mail
Tel.: +49 381 494 9337

Prof. Dr.-Ing. Hermann Seitz
E-Mail
Tel.: +49 381 498 9090

Institutions

Rostock University Medical Center
Department of Orthopaedics
Doberaner Straße 142
18057 Rostock

University of Rostock
Chair of Mikrofluidics
Justus-von-Liebig Weg 6
18059 Rostock

Fellowship holders

Mareike Specht (finished)