C02 - Electrical and mechanical stimulation of hyaline cartilage: Characterisation of biological response and stimulation parameters

Summary of the work programme

Within this project, the influence of electrical and mechanical stimulation on chondrogenic differentiation of human cartilage cells and mesenchymal stem cells will be analysed in vitro. For this purpose, we will use a specific stimulation chamber for experimental analyses of single and combined stimulation. Further, we will establish a multiphysics simulation model which should comprise the stimulation parameters and their influence on cells resulting in an enhanced understanding of the stimulation process. In the first funding period, the main goal of C02 is to establish and achieve optimised 3D cultivation of human chondrocytes and mesenchymal stem cells on novel collagen- and hydrogel-based biomaterials (scaffolds) by means of combined electrical and mechanical stimulation to derive effective therapeutic approaches.

Cooperation projects

A02 / A03 / A05 / B01 / B02 / B03 / C01 / C03 / C04

Publications

Abdul R. Farooqi, Julius Zimmermann, Rainer Bader, Ursula van Rienen: Numerical Simulation of Electroactive Hydrogels for Cartilage–Tissue Engineering. Materials 2019, 12(18), 2913, September 2019. doi.org/10.3390/ma12182913

Hendrikje Raben, Peer W. Kämmerer, Rainer Bader and Ursula van Rienen: Establishment of a Numerical Model to Design an Electro-Stimulating System for a Porcine Mandibular Critical Size Defect. Applied Sciences, volume 9(10), May 2019. ISSN 2076-3417. doi.org/10.3390/app9102160

Abdul Razzaq Farooqi, Rainer Bader and Ursula van Rienen: Numerical study on electromechanics in cartilage tissue with respect to its electrical properties. Tissue Engineering Part B: Reviews, April 2019. doi.org/10.1089/ten.teb.2018.0214

Bettina Hiemer, Martin Krogull, Thomas Bender, Josefin Ziebart ,Simone Krueger, Rainer Bader, Anika Jonitz-Heincke: Effect of electric stimulation on human chondrocytes and mesenchymal stem cells under normoxia and hypoxia. Mol Med Rep. 18(2):2133-2141. August 2018. doi.org/10.3892/mmr.2018.9174

Josefin Ziebart, Tina Sellmann, Karin Porath, Bachir Delenda, Ursula van Rienen, Rainer Bader and Rüdiger Köhling: Investigation of calcium channels involved in directed migration of human osteoblast in a direct current field. Orthopaedic Proceedings, volume SUPP_4: pages 4-4, April 2018. online.boneandjoint.org.uk/doi/abs/10.1302/1358-992X.2018.4.004

Daniel Kluess, Hans-E. Lange, Horst Heyer, Manuela Sander, Wolfram Mittelmeier and Rainer Bader: Supplementary  finite element analysis in experimental testing of total hip stems. Materials Testing, volume 60 (5): pages 489-494, April 2018. doi.org/10.3139/120.111182

Josefin Ziebart, Siyuan Fan, Christian Schulze, Peer W. Kämmerer, Rainer Bader and Anika Jonitz-Heincke: Effects of interfacial micromotions on vitality and differentiation of human osteoblasts. Bone & Joint Research, volume 7 (2): pages 187-195, February 2018. doi.org/10.1302/2046-3758.72.bjr-2017-0228.r1

Conference papers

Alina Weizel, Julius Zimmermann, Alexander Riess, Simone Krüger, Rainer Bader, Ursula van Rienen, Hermann Seitz: Numerical Numerical simulation of the electric field distribution in an electrical stimulation device for scaffolds settled with cartilaginous cells. EMBC, July 2019, Berlin, Germany. doi:10.1109/EMBC.2019.8857760

Fabian Möws, Josefine Ziebart, Carolin Gabler, Simone Krüger, Daniel Wolter, Martin Ellenrieder and Rainer Bader: Etablierung eines Tiermodells zur Untersuchung der Osseointegration elektrostimulierender Implantate, VIII. Münchener Symposium für Experimentelle Orthopädie, Unfallchirurgie und Muskuloskelettale Forschung, March 2018, Munich, Germany.

Talks

Rainer Bader: Numerische Simulation in der Biomaterialforschung, Research Workshop, University Medical Center, November 2017, Rostock, Germany.

Posters

Alina Weizel, Thomas Diestler, Christian Polley, Rainer Detsch, Aldo. R. Boccaccini: Mechanical and rheological characterization of hydrogels and hydrogel precursors for cartilage tissue engineering and biofabrication. AMMM + 3D Week, September 2019, Lübeck, Germany.

Yogesh Deepak Bansod, Märuan Kebbach, Rainer Bader, and Ursula van Rienen: A Piezoelectric Strain-Adaptive Bone Remodeling Simulation. 41st International  Engineering in Medicine and Biology Conference (EMBC), July 2019, Berlin, Germany.

Alina Weizel, Thomas Diestler, Christian Polley, Rainer Detsch, Aldo R. Boccaccini, Hermann Seitz: Relaxation Behavior of Ionically and Enzymatically Microbial Transglutaminase (mTG)-Crosslinked Alginate-di-aldehyde and Gelatin (ADA-GEL) Hydrogels for Cartilage Regeneration, 4th Euro Intelligent Materials, June 2019, Kiel, Germany.

Project leader

Prof. Dr. med. habil. Dipl.-Ing. Rainer Bader
E-Mail
Tel.: +49 381 494 9337

Prof. Dr.-Ing. Hermann Seitz
E-Mail
Tel.: +49 381 498 9090

Institutions

Rostock University Medical Center
Department of Orthopaedics
Doberaner Straße 142
18057 Rostock

University of Rostock
Chair of Mikrofluidics
Justus-von-Liebig Weg 6
18059 Rostock

Fellowship holders

Mareike Specht (finished)