Summary of the work programme

In this project, two cell signalling pathways – Ca2+ and Wnt – under alternating current stimulation in vitro are in focus. The intracellular calcium ion mobilisation in vital human osteoblasts and mesenchymal stem cells will be locally recorded via scanning ion conductance microscopy and confocal laser scanning microscopy. In vitro data of components of the Wnt signalling pathway and of reactive oxygen species will be delivered for modelling and simulation in silico. A decisive step is the preparation of electrode patterns to be covered by polymers. The potential at the electrodes will be independently controlled allowing for the assessment of lamellipodia formation or membrane ruffle alterations towards specific electric signals or patterns.