A03 - Material surface charges and their influence on cell physiology and morphology

Summary of the work programme

This Project addresses the relation and mechanisms of interaction between osteoblasts and titanium surfaces, in particular the role of material surface charges on cell behaviour. The project will study how structural, chemical, and electric parameters at the interface to the bio-system and their interplay govern cellular physiology (i.a. calcium signalling) and morphology (i.a. the migration front zone via Scanning Ion Conductance-Microscopy). To this end, methods of surface functionalisation, structuring, nanoprobing, and cell biology will be combined.

Cooperation projects

A01 / A02 / A04 / B01 / B05 / C01 / C02 / C04

Publications

2020

Rebl, Henrike; Renner, Jürgen; Kram, Wolfgang; Springer, Armin; Fritsch, Nele; Hansmann, Harald et al. (2020): Prevention of Encrustation on Ureteral Stents: Which Surface Parameters Provide Guidance for the Development of Novel Stent Materials? In: Polymers 12 (3), pp. 1-16, 558. DOI: 10.3390/polym12030558.
 

2019

Bergemann, Claudia; Rebl, Henrike; Otto, Anik; Matschke, Svenja; Nebe, Barbara J. (2019): Pyruvate as a cell‐protective agent during cold atmospheric plasma treatment in vitro: Impact on basic research for selective killing of tumor cells. In: Plasma Processes and Polymers 59, S. 14031. DOI: 10.1002/ppap.201900088.

Habets, Thomas; Lensen, Dennis; Speller, Sylvia; Elemans, Johannes A. A. W. (2019): Self-Assembly of Covalently Linked Porphyrin Dimers at the Solid-Liquid Interface. In: Molecules 24 (16). DOI: 10.3390/molecules24163018.

Nebe, Barbara J.; Rebl, Henrike; Schlosser, Michael; Stählke, Susanne; Grüning, Martina; Weltmann, Klaus-Dieter et al. (2019): Plasma Polymerized Allylamine-The Unique Cell-Attractive Nanolayer for Dental Implant Materials. In: Polymers 11 (6). DOI: 10.3390/polym11061004.

Oldenburg, Kevin; Hartmann, Hannes; Lermé, Jean; Pohl, Marga-Martina; Meiwes-Broer, Karl-Heinz; Barke, Ingo; Speller, Sylvia (2019): Virtual Plasmonic Dimers for Ultrasensitive Inspection of Cluster–Surface Coupling. In: The Journal of Physical Chemistry 123 (2), S. 1379–1388. DOI: 10.1021/acs.jpcc.8b08928.

Rohr, Nadja; Nebe, Barbara J.; Schmidli, Fredy; Müller, Petra; Weber, Michael; Fischer, Horst; Fischer, Jens (2019): Influence of bioactive glass-coating of zirconia implant surfaces on human osteoblast behavior in vitro. In: Dental materials 35 (6), S. 862–870. DOI: 10.1016/j.dental.2019.02.029.

Schnell, Georg; Stählke, Susanne; Duenow, Ulrike; Nebe, Barbara J.; Seitz, Hermann (2019): Femtosecond Laser Nano/Micro Textured Ti6Al4V Surfaces-Effect on Wetting and MG-63 Cell Adhesion. In: Materials 12 (13). DOI: 10.3390/ma12132210.

Schröder, Max; Stählke, Susanne; Nebe, Barbara J.; Krüger, Frank (2019): SFB-ELAINE/Biomedical-Jupyter-Examples: v1.0. In: Python. DOI: 10.5281/zenodo.3548652.

Stählke, Susanne; Lehnfeld, Jutta; Schneider, Andreas; Nebe, Barbara J.; Müller, Rainer (2019): Terminal chemical functions of polyamidoamine dendrimer surfaces and its impact on bone cell growth. In: Materials Science and Engineering: C 101, S. 190–203. DOI: 10.1016/j.msec.2019.03.073.
 

2018

Schnabelrauch, Mathias; Dubs, Manuela; Kautz, Armin R.; Weisser, Jürgen; Bergemann, Claudia; Rebl, Henrike;  Schmidt, Jürgen; Witt, Carolin; Oberbach, Thomas; Imhof, Diana; Nebe, Barbara (2018): Biofunctionalization of Ceramic Implant Surfaces to Improve their Bone Ingrowth Behavior. In: Materials Science Forum 941, S. 2483–2488. DOI: 10.4028/www.scientific.net/msf.941.2483.

Stählke, Susanne; Rebl, Henrike; Finke, Birgit; Müller, Petra; Grüning, Martina; Nebe, Barbara J. (2018): Enhanced calcium ion mobilization in osteoblasts on amino group containing plasma polymer nanolayer. In: Cell & Bioscience 8 (1), S. 22. DOI: 10.1186/s13578-018-0220-8.

Stählke, Susanne; Rebl, Henrike; Nebe, Barbara J. (2018): Phenotypic stability of the human MG-63 osteoblastic cell line at different passages. In: Cell Biology International 43 (1), S. 22–32. DOI: 10.1002/cbin.11073.

Truong, Duy; Bahls, Christian R.; Nebe, Barbara J.; van Rienen, Ursula (2018): Cover Image. In: International Journal for Numerical Methods in Biomedical Engineering 34 (8), e3135. DOI: 10.1002/(ISSN)2040-7947.

Truong, Duy; Bahls, Christian R.; Nebe, Barbara J.; van Rienen, Ursula (2018): Simulation of actin distribution of osteoblasts on titanium pillar arrays using a bio-chemo-mechanical model. In: International Journal for Numerical Methods in Biomedical Engineering, e3097. DOI: 10.1002/cnm.3097.

Conference papers

2020

Grüning, Martina; Lehnfeld, Jutta; Neuber, Sven; Nestler, Peter; Fricke, Katja; Dubs, Manuela et al. (2020): Zeta potential as key property for the development of functional biomaterials. 11th World Biomaterials Congress. Glasgow, Scotland, May 2020.
 

2019

Nebe, Barbara J. (2019): Chemie und Mikrostruktur – Zellverhalten an der Biomaterial-Grenzfläche. Progress in Science and Education on Ceramics. Zürich, Switzerland, September 2019.

Nebe, Barbara J.; Grüning, Martina; Rebl, Henrike; Finke, Birgit; Helm, Christiane A.; Schnabelrauch, Matthias et al. (2019): Complex cell physiology on chemically and topographically designed implant surfaces. 3rd International Conference on Dentistry and Oral Health. London, United Kingdom, September 2019.

Nebe, Barbara J.; Stählke, Susanne; Rebl, Henrike; Mörke, Caroline; Grüning, Martina (2019): Zellaktivität an der Keramik-Grenzfläche. 3. Kongress der Deutschen Gesellschaft für Implantologie im Zahn-, Mund- und Kieferbereich e.V. Hamburg, Germany, November 2019.

Rebl, Henrike; Renner, Jürgen; Zacharias, Bernd; Kram, Wolfgang; Springer, Armin; Weisser, Jürgen et al. (2019): Prevention of encrustation and biofilm formation on ureteral catheters - which surface parameters are directive for the development of novel catheter materials? 30th Annual Conference of the European Society for Biomaterials. Dresden, Germany, September 2019.

Stählke, Susanne; Grüning, Martina; Zimmermann, Julius; Freitag, Thomas; Prokopp, Alexander; Barke, Ingo et al. (2019): Osteoblast behavior upon electrical stimulation in vitro. Annual Conference of the European Society for Biomaterials. Dresden, Germany, September 2019.

Stählke, Susanne; Mazur, Karolina; Krężel, Aleksandra; Żydek, Jagoda; Pamula, Elzbieta; Pietryga, Krzysztof et al. (2019): Whey protein isolate hydrogels as scaffolds for bone regeneration. 30th Annual Conference of the European Society for Biomaterials. Dresden, Germany, September 2019.

Völkner, Christian; Lange, Regina; Grüning, Martina, Fricke, Katja; Assi, Issam; Barke, Ingo; Nebe, Barbara J.; Speller, Sylvia (2019): How can live-cell nanoprobing help to unveil osteoblast adhesion properties? 30th Annual Conference of the European Society for Biomaterials. Dresden, Germany, September 2019.
 

2018

Grüning, Martina; Rebl, Henrike; Stählke, Susanne; Finke, Birgit; Neuber, Sven; Helm, Christiane A.; Nebe, Barbara J. (2018): Biomaterial surface charges and their influence on cell signaling in living osteoblasts. 29th European Conference on Biomaterials. Maastrict, Netherlands, September 2018.

Grüning, Martina; Rebl, Henrike; Stählke, Susanne; Finke, Birgit; Neuber, Sven; Helm, Christiane A.; Nebe, Barbara J. (2018): Polyelectrolyte multilayers on titanium and their influence on osteoblast behavior. 9th International Congress Nanotechnology in Biology Medicine. Graz, Austria, April 2018.

Nebe, Barbara J. (2018): Bone cells at the interface - influence of topography and chemistry. 2nd Sino-German Symposium on Reconstruction and Repair of Musculoskeletal Tissues. Mainz, Germany, March 2018.

Rebl, Henrike (2018): Optimized cell-biomaterial contact due to plasma modificatio. Energy Materials and Nanotechnology. Kuala Lumpur, Malaysia, January 2018.
 

2017

Nebe, Barbara J. (2017): Bioactivation of implant materials and induction of cell signaling via a plasma polymer nanolayer. 28th European Society of Biomaterials. Athens, Greece, September 2017.

Stählke, Susanne (2017): Biomaterial modifications and cellular behaviour. 26th Annual Conference Biomaterials in Medicine and Veterinary Medicine. Rytro, Poland, November 2017.

Stählke, Susanne (2017): Intracellular calcium dynamics dependent on defined features of titanium materials. Fimpart Enabling Innovation: Frontiers in Materials Processing Applications, Research and Technology. Bordeaux, France, July 2017.

Talks

2019

Nebe, Barbara J. (2019): Cell attractive design of material surfaces – effects of topography and chemistry. University of Erlangen-Nuremberg. Erlangen, Germany, January 2019.

Staehlke, Susanne: Topographical influence of material surfaces on cell morphology. Graduiertenworkshop, 6. Treffen des Graduiertennetzwerkes Life, Light and Matter. Rostock, Germany, January 2019.
 

2018

Nebe, Barbara J. (2018): Bone cells at the interface - influence of topography and chemistry. 2nd Sino-German Symposium on Reconstruction and Repair of Musculoskeletal Tissues. Mainz, Germany, March 2018.

Nebe, Barbara J. (2018): Biomaterial surface charges and their influence on cell behavior and signaling. International Conference on Processing & Manufacturing of Advanced Materials. Paris, France, July 2018.

Nebe, Barbara J. (2018): Cell-Biomaterial Interface - Nanocoating Dominates Microtopography. BIT’s 8th Annual Congress of Nano Science and Technology. Potsdam, Germany, October 2018.

Stählke, Susanne (2018): Topographical influence of material surfaces on cell morphology. Graduiertenworkshop, 6. Treffen des Graduiertennetzwerkes Life, Light and Matter. Rostock, Germany, January 2018.

Stählke, Susanne (2018): Intracellular calcium ion signaling dependent on surface properties of biomaterials. 3rd Annual Conference and Expo on Biomaterials. Berlin, Germany, March 2018.
 

2017

Rebl, Henrike (2017): Interaktion von Zellen an der Grenzfläche zu chemisch modifizierten Biomaterialoberflächen. University Medical Center. Rostock, Germany, November 2017.

Stählke, Susanne (2017): Intracellular calcium dynamics dependent on defined features of titanium materials. Fimpart Enabling Innovation: Frontiers in Materials Processing Applications, Research and Technology. Bordeaux, France, July 2017.

Posters

2019

Engster, Katharina; Oldenburg, Kevin; Völkner, Christian; Lermé, Jean; Elemans, JAAW; Meiwes-Broer, Karl-Heinz et al. (2019): Locally excited electron systems in nanoparticle and molecule aggregate landscapes. University of Rostock. Rostock, Germany.

Grüning, Martina; Lehnfeld, Jutta; Müller, Petra; Fricke, Katja; Neuber, Sven; Helm, Christiane A. et al. (2019): The role of material’s zeta potential on intracellular calcium ion signaling. 4th European Symposium on Intelligent Materials. Kiel, Germany.

Grüning, Martina; Lehnfeld, Jutta; Müller, Rainer; Fricke, Katja; Neuber, Sven; Helm, Christiane A. et al. (2019): Material surface charges and their influence on cell physiology. 6th International Symposium Interface Biology of Implants. Warnemünde, Germany.

Stählke, Susanne, Grüning Martina Zimmermann, Julius Freitag, Thomas Prokopp, Alexander Barke, Ingo Speller, Sylvia van Rienen, Ursula Nebe, Barbara J. (2019): Osteoblast behavior upon electrical stimulation in vitro. University of Rostock. Rostock, Germany.
 

2018

Bahls, Christian R.; Truong, Duy; Nebe, Barbara J.; van Rienen, Ursula (2018): Modelling contraction of biological cells under electric field exposure using a bio-chemo-mechanical model. 11th International Symposium on Electric and Magnetic Fields. Darmstadt, Germany.

Grüning, Martina; Rebl, Henrike; Stählke, Susanne; Finke, Birgit; Neuber, Sven; Helm, Christiane A.; Nebe, Barbara J. (2018): Polyelectrolyte multilayers on titanium and their influence on osteoblast behavior. Graz, Austria.

Grüning, Martina; Rebl, Henrike; Stählke, Susanne; Finke, Birgit; Neuber, Sven; Helm, Christiane A.; Nebe, Barbara J. (2018): Biomaterial surface charges and their influence on cell signaling in living osteoblasts. 29th European Conference on Biomaterials. Maastricht, Netherlands.

Nebe, Barbara J.; Rebl, Henrike; Finke, Birgit; Mörke, Caroline; Grüning, Martina; Schnabelrauch, Matthias; Stählke, Susanne (2018): Cell-Biomaterial Interface - Nanocoating Dominates Microtopography. BIT’s 8th Annual Congress of Nano Science and Technology. Potsdam, Germany.

Nebe, Barbara J.; Stählke, Susanne; Schnabelrauch, Matthias; Finke, Birgit; Anselme, Karine; Mörke, Caroline et al. (2018): Biomaterial surface charges and their influence on cell behavior and signaling. 10th International Conference on Processing & Manufacturing of Advanced Materials. Evry, France.

Stählke, Susanne; Rebl, Henrike; Grüning, Martina; Nebe, Barbara J. (2018): Intracellular calcium ion signaling dependent on surface properties of biomaterials. International 3rd Annual Conference and Expo On Biomaterials. Berlin, Germany.

Völkner, Christian; Grüning, Martina; Wendt, Mirco; Rebl, Henrike; Lange, Regina; Nebe, Barbara J.; Speller, Sylvia (2018): Material-Oberflächenladungen und ihr Einfluss auf die Zell-Physiologie und –Morphologie. University of Rostock. Rostock, Germany.

Völkner, Christian; Wendt, Mirco; Lange, Regina; Vogler, Björn; Grüning, Martina; Rebl, Henrike et al. (2018): Scanning Ion Conductance Microscopy on osteoblasts with regard to their adhesion on surfaces. Frühjahrstagung der Deutschen Physikalischen Gesellschaft. Berlin, Germany.